Journal: Frontiers in Microbiology

Article Title: Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production

doi: 10.3389/fmicb.2021.745299

Figure Lengend Snippet: The Lacticaseibacillus casei -conditioned medium (LCM) of L. casei T21 attenuated C. difficile -induced IL-8 production, host gene expression, and increased transepithelial electrical resistance of C. difficile -stimulated colonic epithelial cells. The results are shown as IL-8 production in Caco-2 and HT-29 cells, respectively (A,B) ; IL-8 gene expression (relative to GAPDH ) in Caco-2 and HT-29 cells, respectively (C,D) ; the expression of SLC11A1 , HuR , and MUC-2 in Caco-2 cells, respectively (E–G) ; the expression of SLC11A1 , HuR , and MUC-2 in HT-29 cells, respectively (H–J) ; the transepithelial electrical resistance (TEER) values of Caco-2 cells (K) ; and the pH of cell culture medium (McCoy’s 5A modified medium for HT-29 cells) (L) . The results were from three independent experiments each in triplicate and expressed as the mean ± SEM. *p < 0.05.

Article Snippet: Colonic epithelial cells were then incubated with viable cells of C. difficile ATCC BAA1870 at multiplicity of infection (MOI) 1:300 either alone or with 5% (vol/vol) LCM for 24 h in 5% CO 2 at 37°C.

Techniques: Gene Expression, Expressing, Cell Culture, Modification

Journal: PLoS ONE

Article Title: CD133 Expression Is Not Synonymous to Immunoreactivity for AC133 and Fluctuates throughout the Cell Cycle in Glioma Stem-Like Cells

doi: 10.1371/journal.pone.0130519

Figure Lengend Snippet: A. Representative flow cytometry histograms of CaCo-2 cells immunolabeled with anti-AC133 Ab ( left panel ) or anti-CD133CT Ab ( right panel ). B. Quantification of AC133- and CD133CT-positive cells from three independent experiments. Bars represent mean percentage of positive cells ± SEM. C. Immunofluorescence microscopy of CaCo-2 cells co-stained with anti-AC133 Ab (green) and anti-CD133CT Ab (red). Nuclei were counterstained using DAPI (blue). Confocal microscopy images ( inset ) show the subcellular distribution of the staining. Merged image shows overlapping signals generated by anti-AC133 Ab ( green ) and anti-CD133CT Ab ( red ) antibodies. Scale bars correspond to 50 μm (main fields) and 7 μm (insets).

Article Snippet: The human colon carcinoma cell line CaCo-2 [ ] was a kind gift from Dr. Frauke Alvez (University Medical Center, Göttingen) and was obtained from the German collection for microorganims and cell cultures (DSMZ, No. ACC169).

Techniques: Flow Cytometry, Immunolabeling, Immunofluorescence, Microscopy, Staining, Confocal Microscopy, Generated

Journal: PLoS ONE

Article Title: CD133 Expression Is Not Synonymous to Immunoreactivity for AC133 and Fluctuates throughout the Cell Cycle in Glioma Stem-Like Cells

doi: 10.1371/journal.pone.0130519

Figure Lengend Snippet: A. Western blot analysis of crude lysates (100 μg total protein per lane) from mock-treated CaCo-2 cells (lanes 1) or CaCo-2 cells transfected with the validated anti-CD133 siRNA (LifeTechnologies) (lanes 2–4). After the transfer to the PVDF membrane, Ponceau S staining was done to verify the efficacy of protein transfer ( top panel ) and followed by sequential probing with anti-AC133 Ab ( middle panel ) and anti-CD133CT Ab ( bottom panel ), respectively. B. Comparative assessment of CD133 in unfractionated cell lysates (“input”, 20 μg) and plasma membranes (“PM”, 1 μg) by anti-AC133 or anti-CD133CT Abs.

Article Snippet: The human colon carcinoma cell line CaCo-2 [ ] was a kind gift from Dr. Frauke Alvez (University Medical Center, Göttingen) and was obtained from the German collection for microorganims and cell cultures (DSMZ, No. ACC169).

Techniques: Western Blot, Transfection, Membrane, Staining, Clinical Proteomics

Journal: PLoS ONE

Article Title: CD133 Expression Is Not Synonymous to Immunoreactivity for AC133 and Fluctuates throughout the Cell Cycle in Glioma Stem-Like Cells

doi: 10.1371/journal.pone.0130519

Figure Lengend Snippet: A. Representative histograms showing CD133 surface expression detected by anti-AC133 Ab ( left panels ) or anti-CD133CT Ab ( right panels ) in primary GSCs cultures and stem-like glioma clone G112SP. B. Mean percentage of cells positively labelled with anti-AC133 Ab ( blue ) or anti-CD133CT Ab ( black) in a panel of GSC lines and CaCo-2 cells used as a positive control. C. Comparative assessment of the total (“input”) and membrane-associated (“PM”) CD133 protein in human GSC lines No. 1063, No. 1080 and No. 1051. 20 μg of proteins were loaded per lane. D. Representative histograms showing surface expression of CD133/2 detected by anti-AC141 Ab in primary cultures of GSCs, stem-like glioma clone G112SP and reference cell line CaCo-2 used as a positive control.

Article Snippet: The human colon carcinoma cell line CaCo-2 [ ] was a kind gift from Dr. Frauke Alvez (University Medical Center, Göttingen) and was obtained from the German collection for microorganims and cell cultures (DSMZ, No. ACC169).

Techniques: Expressing, Positive Control, Membrane